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Registro Completo |
Biblioteca(s): |
Epagri-Sede. |
Data corrente: |
09/04/2002 |
Data da última atualização: |
09/04/2002 |
Autoria: |
BORTOLUZZI-MAAG, G.; BRACKMANN, A. |
Título: |
Qualidade da maca fuji relacionada com a umidade relativa e a temperatura de armazenamento. |
Ano de publicação: |
0 |
Fonte/Imprenta: |
Revista Cientifica Rural, Bage,RS, v.6, n.1, p.1-5, 2001. |
Idioma: |
Português |
Palavras-Chave: |
Armazenamento; Atmosfera controlada; Conservacao; Maca; Polpa. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00526naa a2200181 a 4500 001 1020971 005 2002-04-09 008 bl uuuu u00u1 u #d 100 1 $aBORTOLUZZI-MAAG, G. 245 $aQualidade da maca fuji relacionada com a umidade relativa e a temperatura de armazenamento. 260 $c0 653 $aArmazenamento 653 $aAtmosfera controlada 653 $aConservacao 653 $aMaca 653 $aPolpa 700 1 $aBRACKMANN, A. 773 $tRevista Cientifica Rural, Bage,RS$gv.6, n.1, p.1-5, 2001.
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Registro Completo
Biblioteca(s): |
Epagri-Sede. |
Data corrente: |
10/09/2020 |
Data da última atualização: |
10/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - B |
Autoria: |
SOUZA, J. A.; BOGO, A.; BETTONI, J. C.; COSTA, M. D.; SILVA, F. N.; CASA, R. T.; RUFATO, L. |
Título: |
Droplet-vitrification cryotherapy for eradication of apple stem grooving virus and apple stem pitting virus from ?Marubakaido? apple rootstock. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 45, n. , p. 148-152, 2020. |
Idioma: |
Inglês |
Conteúdo: |
The apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV) are among the major viral species that infect apple trees and are responsible for substantial damage and loss. The aim of this study was to assess the effectiveness of droplet-vitrification cryotherapy technique in eradication of ASGV and ASPV in Malus prunifolia ?Marubakaido? apple rootstock plants. The axillary shoot tips were excised from ASGV- and ASPV-infected plants and incubated for one day on preculture medium. Subsequently, they were exposed to plant vitrification solution 2 (PVS2) for 0, 20, 40, 50, 60 or 80 min at 22 °C and immersed in liquid nitrogen for 1 h. Cryopreserved shoot tips had the highest survival (75 and 65%) and regrowth (53 and 58%) percentages with PVS2 exposure duration of 20 and 40 min, respectively. After six months of growth in a greenhouse, RT-PCR analyses revealed that all the tested plants were free of ASPV and 70% were free of ASGV. The high eradication percentage of ASGV and ASPV indicated that the droplet-vitrification cryotherapy technique is an effective tool to produce ASGV- and ASPV-free propagating material of ?Marubakaido? apple rootstock. |
Palavras-Chave: |
Cryopreservation; Malus prunifolia; Propagation; Virus-free material. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01926naa a2200241 a 4500 001 1129905 005 2020-09-10 008 2020 bl uuuu u00u1 u #d 100 1 $aSOUZA, J. A. 245 $aDroplet-vitrification cryotherapy for eradication of apple stem grooving virus and apple stem pitting virus from ?Marubakaido? apple rootstock.$h[electronic resource] 260 $c2020 520 $aThe apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV) are among the major viral species that infect apple trees and are responsible for substantial damage and loss. The aim of this study was to assess the effectiveness of droplet-vitrification cryotherapy technique in eradication of ASGV and ASPV in Malus prunifolia ?Marubakaido? apple rootstock plants. The axillary shoot tips were excised from ASGV- and ASPV-infected plants and incubated for one day on preculture medium. Subsequently, they were exposed to plant vitrification solution 2 (PVS2) for 0, 20, 40, 50, 60 or 80 min at 22 °C and immersed in liquid nitrogen for 1 h. Cryopreserved shoot tips had the highest survival (75 and 65%) and regrowth (53 and 58%) percentages with PVS2 exposure duration of 20 and 40 min, respectively. After six months of growth in a greenhouse, RT-PCR analyses revealed that all the tested plants were free of ASPV and 70% were free of ASGV. The high eradication percentage of ASGV and ASPV indicated that the droplet-vitrification cryotherapy technique is an effective tool to produce ASGV- and ASPV-free propagating material of ?Marubakaido? apple rootstock. 653 $aCryopreservation 653 $aMalus prunifolia 653 $aPropagation 653 $aVirus-free material 700 1 $aBOGO, A. 700 1 $aBETTONI, J. C. 700 1 $aCOSTA, M. D. 700 1 $aSILVA, F. N. 700 1 $aCASA, R. T. 700 1 $aRUFATO, L. 773 $tTropical Plant Pathology, Brasília$gv. 45, n. , p. 148-152, 2020.
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